#genetransfer
DNA microinjection | Embryonic stem cell mediated gene transfer | Gene transfer techniques

DNA microinjection is the dominating technique leading to random integration of a transgene via the introduction of DNA into the pronucleus of a developing zygote. Following fertilization of a mouse egg, the male and female pronuclei remain separated for a few hours before they fuse to make the zygotic nucleus, thus allowing for microinjection of the desired genes into the larger male pronucleus. Eggs that can survive the injections are transferred into the oviducts of a foster mother i.e., pseudopregnant female mice for the generation of Founder mouse, from which permanent transgenic lines can be established. The presence of transgenes in the offsprings can be identified by PCR analysis or Southern blot hybridization.

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Electroporation
Gene transfer techniques
Dna
Rna
Transcription
Translation
Proteins
Animal biotechnology
Animal transgenesis
Alampally phanindra
Phanindra gupta
molecular biology
dna microinjection method
microinjection method of gene transfer in plants
dna microinjection transgenic animals
microinjection gene transfer method
embryonic stem cell mediated gene transfer
microinjection gene transfer method animation