#chromatography
Column chromotography | Principle, Technique, procedure, applications | Bio science
Column chromatography is frequently used by organic chemists to purify liquids (and solids.) An impure sample is loaded onto a column of adsorbant, such as silica gel or alumina. An organic solvent or a mixture of solvents (the eluent) flows down through the column. Components of the sample separate from each other by partitioning between the stationary packing material (silica or alumina) and the mobile eluent. Molecules with different polarity partition to different extents, and therefore move through the column at different rates. The eluent is collected in fractions.
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